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316. Error? Staining? Centrifugal concentration?

Sarah obviously just arrived in Africa not long ago and had learned about relevant parasite knowledge but was not in-depth. She thought that everything would be fine if she could find microfilariae. However, what she didn't know was that even if the same type of parasites were of the same type, the drugs needed to take were different, otherwise the praziquantel that Lin Zhixing had fed her before should be effective.

"Are there any microscope photos?" Qi Jing asked.

It was indeed the same as Huang Xinghua thought. After hearing this, Sara shook her head and said helplessly: "We only have the most ordinary optical microscopes here, and the laboratory department does not have such advanced imaging equipment."

Since there is no photo, Qi Jing wants to start from the person: "Is the doctor who is doing the examination here? I want to ask him some questions."

"The doctors in the laboratory department here have not been enough, and we can only do some tests ourselves." Sara pointed at herself with her fingers, as if she was a little proud of her polygonal ability. "I did both Dr. Lin's blood smear and bone marrow smear tests, and I'm used to it anyway."

The lack of medical treatment and medicine in the local area is also an objective fact. Qi Jing did not get the advantages he wanted to express. Instead, he sighed and asked, "Then didn't you let Dr. Lin take a look?"

"Doctor Lin?" Sarah shook her head, "He went to check after the examination, so I did it alone."

Qi Jing also frowned and questioned: "You have no experience in parasites, it doesn't matter. But when you are doing the examination, especially when you see the insect body, should you find an experienced Dr. Lin to take a look at the results and do a simple parasite morphology identification?"

Qi Jing's tone was not light. After hearing this, Sarah was silent for a while before convinced herself. In this matter, she was indeed greedy and a little arrogant.

In terms of parasites, Sara has limited skills, but she only studied tropical diseases for half a month when she came to Africa. She has a rough understanding of common infectious diseases in Africa, such as malaria, myiasis, filaria, and various viruses.

But once you dig into some details, it won't work.

However, Sarah has been the attending physician who has been working for more than ten years in clinical work. She felt uncomfortable when she was told by a young doctor who seemed to be several years younger than her.

In order to save face, she could only defend herself and wanted to get a step forward:

"Because we found nothing in the smear I did before, we specially looked for Dr. Lin before the examination. He suggested that we change to centrifugal concentration and finally use a 40x high-power microscope for examination. Then we found two microfilariae. At that time, he didn't say that we would look at the results together. Who would have thought that we would leave an image for the examination..."

After hearing this, Huang Xinghua chuckled, and then gave Qi Jing a special wink: "Forget it."

Qi Jing didn't say anything, but the directors below couldn't help but laugh and scolded: "What's up? Someone else said something for half a minute, but you just turned it over and said 'Forget it'?"

"Lao Huang, you have to be corrected. This is a consultation!"

"If this happens, this consultation cannot continue!"

“It’s amazing to be French!?”

Huang Xinghua slapped at the table: "I'm talking to Qi Jing, why are you in the mouth? I want me to translate this and that. She said that she would do centrifugal concentration for peripheral blood. How many of you can understand? I'm afraid 2/3 are all confused. I have to explain to you what centrifugal concentration means?"

This time, he grabbed the handle of these old men and women, and won the battle with a common inspection method for parasites.

Cai Ping and several infectious diseases experts naturally knew what centrifugal concentration was, but due to Huang Xinghua's sight, he still didn't dare to speak out. Huang Xinghua also said it more and more happily: "It would be nice to let you participate and open your eyes. According to my previous temper, you old guys have to get out!"

When it came to this point, Lao Zhang got up and smoothed out: "Forget it, everyone is old, what's the point of being quarrel? It's rare to get together. If you don't understand, you'll understand if you ask Xiao Qi later? Why bother to get angry with him?"

"Yes, Lao Zhang is right."

"Well, I'll ask Xiao Qi to tell us the details later."

In fact, this episode should be almost over here. Although the senior directors have a bad temper, they are usually half a friend. They often meet during medical seminars, and it is not a good thing to have a broken relationship.

But who knew that someone in the audience suddenly said: "Isn't it just a commonly used examination method in infectious diseases? It's a centrifuge to gather white blood cells and microorganisms in the blood, so that the detection rate can be improved. I thought it was an amazing method, but that's all..."

A stone caused a thousand waves, and the people who had just stopped talking started arguing.

Huang Xinghua's face thrust, and his anger gradually became full. Just as the attack was about to occur, Qi Jing stood up: "Hey, I'm still consulting! You guys..."

He almost said the word "Immortality" and finally stopped it in his throat in time and did not release it. After a moment of pause, Qi Jing quickly added the second half of the sentence, which made people less awkward: "Professors, can you make a noise when I finish the consultation here?"

In fact, these experts are just a person who can persuade them to fight. It would be better if Qi Jing spoke.

But the strange pause just now made them feel uncomfortable. And it happened to be behind the word "old" of "old" in "old". Combined with the tone at the beginning, I always felt something was wrong.

Qi Jing can't have that much, so he focused on the smear examination as soon as possible. Now that there is no image, I can only settle for the second best and ask about other feasibility methods: "Doctor Sarah, are Dr. Lin's two smear specimens still there?"

Sara shook her head: "The operating table is too small. Once I finish it, I have to replace people for other inspections, so these smears have to be thrown away on the spot."

"After discovering microfilariae, have you left an image record?"

Huang Xinghua was stunned for a while after hearing this sentence, but he didn't understand Qi Jing's meaning, but he still translated it as it was.

Qi Jing's question did not follow the ordinary path and deviated from Sarah's established thinking. Just looking at her embarrassed expression, Qi Jing already knew the answer: "Mirror examination of parasites is a very high false negative test. Once the insect body is found, the image must be retained as soon as possible, otherwise there is no way to identify insect species."

Sara was helpless and said quickly: "I have said before that the microscopes here do not have an image acquisition system, so it is impossible to leave an inspection image."

"You can't do things without electronic instruments?"

In terms of his own profession, Qi Jing has always been impatient and has the most strength. Especially when the other party is not strong and does not admit defeat, it is more likely to get angry.

Sarah's rebuttal made him accidentally reveal his usual tone. Qi Jing immediately realized the other party's identity and quickly coughed twice, retracting his temper: "When I first used to send out the micro mirror, I didn't have a camera. When I checked, I would draw the images on paper for archives and records."

After hearing this, Sara suddenly realized: "What should I do now?"

There is no microscopic imaging technology, no professional parasite doctors are there to observe and record, and there is not even a sketch drawn by hand. Qi Jing, a clever woman, has to bite the bullet even if she can't do it.

"Centrifugal concentration, centrifugal concentration..."

Sarah realized her mistake and before Qi Jing came up with a solution, she said, "I'm going to the garbage dump to find those two smears. I made a simple fix before checking it, so I should be able to see clearly."

Seeing that she was about to put down the report paper and was about to turn off the video, Qi Jing quickly stopped her: "You can also do that kind of medical garbage dump? What should I do if I get infected after cutting my skin?"

Qi Jing paused for a moment, thought for a while and asked, "There is no original and no record, so can you describe what form it is?"

Huang Xinghua smiled awkwardly: "Do you want her to tell the truth?"

"What else?" Qi Jing was also helpless.

However, Sarah couldn't tell what image she was seeing. Just because she found a thin strip of stained patterns in a ball of white blood cells, she decided it was microfilariae: "Did I see it wrong?"

"No, that's microfilariae, you read that right." Qi Jing said, "I want to know the sheath of the insect body, the body tail nucleus, and the head gap..."

Huang Xinghua used a question to make a reply: "You are asking a little deeper, she doesn't understand."

Qi Jing's content was getting deeper and deeper, and it had long exceeded Sarah's knowledge and could not understand at all. It was strange to say that a Frenchman could not understand French that had been used for decades, which made Sarah very embarrassed. When this embarrassment was reflected in her, she kept shaking her head: "I can't see anything clearly, it's just a very obvious line."

"I can't see all the internal details?"

"I can't see clearly, I can't see clearly..." Sara suddenly thought of something and asked, "Is it a problem of dyeing?"

This is a common mistake in the laboratory. Due to excessive staining, it covers the original layered structure. Qi Jing could not deny that there was such a possibility, so he asked casually: "Did you use Ruishi dye?"

"No, I used Gisam dyeing." Sara said, "I can only see the solid insect body, and I can't see anything else clearly. Should I change the dye?"

"Not needed for the time being." Qi Jing thought for a while and explained, "Because after centrifugation, white blood cells will stick to the parasite body, increasing the chance of detecting microfilariae. But after adhesion, some characteristics of the insect body are covered by broken white blood cells, so this situation occurs."

"Well, so that's what it is..."

Sarah finally understood a little, but she was still a little confused. She really didn't expect that there would be so many tricks for a blood centrifugal concentration. She also started to become "obedient" from the beginning of being a little prejudiced and disgusted with Qi Jing, and gradually became "obedient": "What should I do next?"

"Can you make thick blood diaphragms?" Qi Jing asked.

"This is simple, I will." Sarah nodded.

"It should be too late to find Lin Zhixing to get some peripheral blood." Qi Jing looked at the time and said, "After taking it, go and make a thick and thin blood diaphragm, then continue to stain with Gisam, and finally use a 10x low-power microscope to scan the picture."

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